After an enzyme is mixed with its substrate, the amount of product formed is determined at 10-second intervals for 1 minute. Data from this experiment are shown below.

Time (sec)                     0      10     20      30      40     50      60

Product formed (mg) 0.0  0.25  0.50   0.70   0.80  0.85    0.85

Draw a graph of these data and answer the following questions.

a. What is the initial rate of this enzymatic reaction?

b. What is the rate after 50 seconds? Why is it different from the initial rate?

c. What would be the effect on product formation if the enzyme were heated to a temperature of 100 ^{O C} for 10 minutes before repeating the experiment? Why?

d. How might altering the substrate concentration affect the rate of the reaction? Why?

e. How might altering the pH affect the rate of reaction? Why?

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